Flow cytometric analysis of M1/M2 surface markers in lipoprotein-treated macrophages.

CD163 (scavenger receptor cysteine-rich type 1 protein M130; hemoglobin/haptoglobin receptor) and CD206 (C-type 1 lectin receptor; mannose receptor) were used as M2 markers while CCR7 (CD197) and CD11c served as M1 markers. Loading with eLDL only induced a statistically significant decrease in CD11c surface expression. On the other hand loading with oxLDL led to significant decreases in CD163 and CD11c, as well as to increases in CCR7 and a not statistically significant decrease in CD206. n = 6, means +/- SD; (*p<0.05, ** p<0.01, *** p<0.001).