Resveratrol induces apoptotic cell death in osteosarcoma cells.

(A) MG-63 and MNNG/HOS cells were treated with 40 μM resveratrol for 48 h. Cells were stained with propidium iodine and Annexin V, then cell apoptosis was analyzed by flow cytometry. (B) Statistics of the apoptosis in vehicle or 40 μM resveratrol treated group as described in A. (C) Activity of caspase 3 was indicated by pNA concentrations. (D) Apoptosis related proteins (cleaved PARP and caspase3, Bax, Bcl-2 and Bcl-xL) were measured with Western blot assays. Resveratrol induces cleavage of PARP and caspase3, upregulation of Bax, and downregulation of Bcl-2 and Bcl-xL. *P < 0.01.