Impact of incorporating dendritic spines on the spatiotemporal spread of signaling microdomains during TBS.

(A) Kymographs showing the spatiotemporal spread of voltage (top) and calcium (middle) and pCaMKII (bottom) microdomains in the spine head, spine neck and dendritic shaft in the presence of 1000 spines on the chosen dendritic oblique, plotted with = 0 mS/cm2 (left) and = 1000 mS/cm2 (right). (B) Peak voltage, Vmax, maximum value of cytosolic calcium concentration, and maximum value of phosphorylated CaMKII concentration, [pCaMKII]max as recorded at the spine head (left), spine neck (center) and dendritic shaft (right) for different values of spines/shaft in the presence of 1000 spines. (C) Peak value (top panels) and area under the curve, AUC (bottom panels) of (left) and [pCaMKII]max (right) plotted as a function of spines/shaft for spine-head (red), spine-neck (blue) and oblique shaft (black). (D) Kymographs showing the spatiotemporal spread of voltage (top) and calcium (middle) and pCaMKII (bottom) microdomains in the spine head, spine neck and dendritic shaft in the presence of 1000 spines on the chosen dendritic oblique, plotted with = 0 mS/cm2 (left) and = 10 mS/cm2 (right). (E) Peak voltage, Vmax, maximum value of cytosolic calcium concentration, and maximum value of phosphorylated CaMKII concentration, [pCaMKII]max as recorded at the spine head (left), spine neck (centre) and dendritic shaft (right) for different values of spines/shaft in the presence of 1000 spines. (F) Peak value (top panels) and area under the curve, AUC (bottom panels) of (left) and [pCaMKII]max (right) plotted as a function of spines/shaft for spine head (red), spine-neck (blue) and oblique shaft (black). All head and neck data shown in this figure correspond to the central synapse-containing spine. All channel conductances were assigned to their default values (Fig 1). Details of parametric values (of active and passive properties in the oblique dendrite shown in Fig 2A) employed for simulations presented in this figure are provided in S1 Table.