Model of striatal medium spiny projection neuron dendrite with spines.

<p>A. Diagram of biochemical signaling pathways. Each arrow is modeled with one or more bimolecular or enzyme reactions. See text and <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002383#pcbi-1002383-t001" target="_blank">Table 1</a> for details. B. Morphology of dendrite with four spines, and location of calcium influx in the model. Subvolumes of height 0.12 µm adjacent to the top and bottom surface of the dendrite are considered submembrane subvolumes. Other dendritic subvolumes are part of the cytosol. Diffusion is two-dimensional in the dendrite and one-dimensional in the spine. C. Experimental Design: The role of anchoring is evaluated using four spatial variations in the location of adenylate cyclase and PKA. The adenylate cyclase-D1R complex (AC) is located either in the spine head or a focal dendritic submembrane area. Similarly, the PKA holoenzyme is located either in the spine head or the focal dendritic submembrane area. AMPA receptors containing GluA1 subunits are in the PSD compartment of the spine head for all cases.