Figure S2. Electrophoresis of primary PCR products after SSH. M: 100 bp marker; Lanes 1–4: products of subtracted first PCR by 62 °C, 64 °C, 66 °C, 68 °C. 10 μM primers and 0.5 U LA Taq polymerase (a), 10 μM primers and 1 U LA Taq polymerase (b), 20 μM primers and 0.5 U LA Taq polymerase (c). (TIF 12107 kb
(A) PCR products encompassing the two break-points shown in Fig. 1 confirm the unitig sequences rath...
Amplification patterns of six 1V and 7V candidate chromosome-specific primers in Chinese (CS), seven...
Figure S4. PCR analysis on a set of nine independent transformants obtained through electroporation ...
Figure S3. Electrophoresis of secondary PCR products after SSH. M: 100 bp marker; Lanes 1–4: product...
Figure S1. Ligation efficiency of different concentrations of digested gDNA. All lanes include AluI ...
Figure S1. Gel electrophoresis of amplification products in TAIL-PCR. Figure S2. Gel electrophoresis...
Figure S11. Gel electrophoresis of 39 primers for real-time PCR with cDNA as template (a and b) and ...
Gel images of PCR amplicons. Gel images of the 13 PCR amplicons used for Tripidium chloroplast isola...
Figure S1. Electrophoresis Gel displaying the DNA fragments created from RT-PCR of (A) E. coli genes...
Amplification and cloning of Pedilanthus leaf curl virus (PeLCV) and Digera yellow vein betasatellit...
Figure S2. Retention of the Luc transgene in a set of Chlamydomonas colonies transformed with the pA...
Figure S1. Molecular identification of transgenic plants. DNA was purified from transgenic and WT pl...
Figure S1. Examples of PCR amplification with the different primer pairs tested. (A) acdsF5/acdsR7; ...
The peak figures of the PCR products sequencing. A, The sequencing peak figures of the PCR products ...
RT-PCR detection of GRSPaV and ubiquitin gene using RNAs isolated from old grapevine leaves with Spe...
(A) PCR products encompassing the two break-points shown in Fig. 1 confirm the unitig sequences rath...
Amplification patterns of six 1V and 7V candidate chromosome-specific primers in Chinese (CS), seven...
Figure S4. PCR analysis on a set of nine independent transformants obtained through electroporation ...
Figure S3. Electrophoresis of secondary PCR products after SSH. M: 100 bp marker; Lanes 1–4: product...
Figure S1. Ligation efficiency of different concentrations of digested gDNA. All lanes include AluI ...
Figure S1. Gel electrophoresis of amplification products in TAIL-PCR. Figure S2. Gel electrophoresis...
Figure S11. Gel electrophoresis of 39 primers for real-time PCR with cDNA as template (a and b) and ...
Gel images of PCR amplicons. Gel images of the 13 PCR amplicons used for Tripidium chloroplast isola...
Figure S1. Electrophoresis Gel displaying the DNA fragments created from RT-PCR of (A) E. coli genes...
Amplification and cloning of Pedilanthus leaf curl virus (PeLCV) and Digera yellow vein betasatellit...
Figure S2. Retention of the Luc transgene in a set of Chlamydomonas colonies transformed with the pA...
Figure S1. Molecular identification of transgenic plants. DNA was purified from transgenic and WT pl...
Figure S1. Examples of PCR amplification with the different primer pairs tested. (A) acdsF5/acdsR7; ...
The peak figures of the PCR products sequencing. A, The sequencing peak figures of the PCR products ...
RT-PCR detection of GRSPaV and ubiquitin gene using RNAs isolated from old grapevine leaves with Spe...
(A) PCR products encompassing the two break-points shown in Fig. 1 confirm the unitig sequences rath...
Amplification patterns of six 1V and 7V candidate chromosome-specific primers in Chinese (CS), seven...
Figure S4. PCR analysis on a set of nine independent transformants obtained through electroporation ...