Additional file 2: Figure S1. of A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

RT-PCR detection of GRSPaV and ubiquitin gene using RNAs isolated from old grapevine leaves with Spectrum™ Plant Total RNA kit (Sigma) with standard method (A and C) or modified method (B and D). (A) and (B): Agarose gel analysis of RT-PCR products amplified with primers RSP35 and RSP36 (Additional file 1: Table S1) on 11 total RNA extracts listed in Table 1 with Method A or B respectively. (C) and (D): Agarose gel analysis of RT-PCR products amplified with UBI primers on these 11 total RNA extracts using Method A and Method B respectively. M: molecular size marker (bp); lane 12: water. (PPT 1035 kb