Additional file 1: of Characterization, phylogeny and recombination analysis of Pedilanthus leaf curl virus-Petunia isolate and its associated betasatellite

Amplification and cloning of Pedilanthus leaf curl virus (PeLCV) and Digera yellow vein betasatellites (DiYVB). (a) Rolling circle amplification (RCA) (Lane 1) and DNA (Lane 2) from petunia leaves, Restriction of RCA product with PstI (Lane 3) and KpnI (lane 4). (b) Restriction confirmation of DiYVB clone in pUC19 with PstI (Lane1 and 2). (c) Restriction confirmation of PeLCV clones with KpnI and BamHI (Lane1, 2, 3). (d) Restriction of RCA products did not yield any required band of 2.8 or 1.4Â kb by digestion with XbaI (lane 1 and 2) and SacI (lane 3 and 4). M-1Â kb marker (Thermo scientific). (DOCX 1347 kb