Additional file 2: of Tonicity inversely modulates lipocalin-2 (Lcn2/24p3/NGAL) receptor (SLC22A17) and Lcn2 expression via Wnt/β-catenin signaling in renal inner medullary collecting duct cells: implications for cell fate and bacterial infection

Figure S2. Verification of hLCN2-R isoform B expression in stable CHO-K1 cell transfectants. (A) Reverse transcription PCR of hLCN2-R-isoform B CHO-K1 clone D6.D10. RNA isolation and cDNA synthesis were carried out as described [31]. Primers and cycling conditions are listed in Table 1. (B) Immunoblot of pcDNA3.1 and hLCN2-R isoform B CHO-K1 cells. Cells were grown to confluency, washed once with PBS, harvested by scraping, and suspended in buffer containing 250mM sucrose, 5 mM Tris, pH7.5, and protease inhibitors. Homogenization by sonication and immunoblotting were carried out as described in the Methods. (PDF 96 kb