Additional file 9: Figure S6. of Lipocalin2 suppresses metastasis of colorectal cancer by attenuating NF-κB-dependent activation of snail and epithelial mesenchymal transition

(A) Quantification of the fold change of luciferase promoter reporter assays of NF-κB in LCN2-knockdown and LCN2-overexpressing cells in 293FT cells. (B,C) p65 and snail (total, nuclear and cytoplasm) expression of SW480 cells treated with LMB (20nM for 10 h) (B) and SW620 cells treated with Bay11-7082 (50 μM for 3 h) (C). (D) Western blots and quantification of EMT markers, p65 (nuclear and cytoplasm) expression in SW620 cells treated with JSH-23 (50 μM for 1 h), (N, nuclear; C-cytoplasm). (E) Survival analysis (Kaplan-Meier method, log-rank test) of LCN2 expression in CRC patients (n = 126, P = .237). (F) Correlation between LCN2/NF-κB expression and nuclear snail expression in clinical samples (group 1: LCN2 (+)/NF-κB (−), total number = 21, 7 cases with positive snail; group 2–4: other groups, total number = 103, 59 cases with positive snail). Values shown are the mean ± SD from at least three independent experiments. * P < .05, ** P < .01, *** P < .001. (TIF 2481 kb