Additional file 2: Figure S2. of Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway

Effects of n-LDL and ox-LDL on cell viability, apoptosis and β-catenin expression in HUVECs. Cells were treated with n-LDL (100 mg/L) or ox-LDL (100 mg/L) for 24 h. a Cell viability was measured by an MTT assay and results were expressed as the percentage of the control. b Cell apoptosis was detected using flow cytometry, and the total apoptotic cells (early and late-stage apoptosis) are presented in the panel (Annexin V-FITC staining alone or together with PI). c Protein levels of β-catenin were evaluated by western blotting. All data are shown as the mean ± SD of at least 3 independent experiments. **P < 0.01 versus the control group. (PDF 237 kb