IL-1+OSM induces sustained expression of CSRNP1 mRNA.

Human chondrocytes were treated with IL-1 (0.05 ng/mL) in combination with OSM (10 ng/mL) for the indicated time points and total RNA isolated. (A) RNA from three separate populations (#1, #2 and #3) was profiled using the Human HT-12v4 Expression Beadchip. The expression profile for CSRNP1 from the microarray dataset for each chondrocyte population is shown. RNA from 6 (B) or 3 (C) separate chondrocyte populations was subjected to qPCR for FOS (C) or CSRNP1 (B and C), and relative expression levels (normalized to 18S rRNA or GAPDH as indicated) at the indicated times determined. The plots show mean ± SD. Data from the populations used in the microarray analyses in (A) are highlighted by the solid symbols (#1, ▲; #2 ■; #3, ●). (C) Statistical comparisons are: ***, p<0.001; **, p<0.01; *, p<0.05 versus basal.