Cortical injury.

Injured Prkdc KO rats display reductions in the thickness of the right S1 (A) while their WT counterparts do not (B). Injured KO rats also display a concomitant reduction in neurons in the right S1 (C) while WT animals do not (D). Representative immunofluorescence in S1 of KO Sham (E top left), KO injured (E bottom left), WT Sham (E top right), and WT injured (E bottom right) in the right and left hemispheres. Where neuronal loss was observed, astrogliosis was evident (green = NeuN, red = GFAP). (F) Landmarking for the S1 measurements was performed from the midline (black arrows) and locations for the S1 thickness measurement (red arrow) and S1 neuronal counts (red boxes) are delineated. Injured Prkdc KO rats display reductions in the thickness of the right M1 (G) while their WT counterparts do not (H). Neither KO (I) nor WT (J) injured animals display neuronal loss in M1. Representative immunofluorescence in M1 of KO Sham (K top left), KO injured (K bottom left), WT Sham (K top right), and WT injured (K bottom right) in the right and left hemispheres (green = NeuN, red = GFAP). (L) Landmarking for the M1 measurements was performed from the peak of the cingulum, and locations for the M1 thickness measurement (red arrow) and M1 neuronal counts (red box) are delineated. Scale bars = 100μm, 20x. Error bars = ±SD. * p<0.05, ** p<0.01, ***p<0.001.