Somatosensory cortex gliosis.

KO injured animals displayed a trend toward elevated stain area occupied by GFAP-positive signal when compared to uninjured controls (A), while WT injured animals did not (B). Representative immunofluorescence is depicted for KO Sham (C top left), KO injured (C bottom left), WT Sham (C top right), and WT injured (C bottom right) in the right and left hemispheres (blue = DAPI, red = GFAP). Percent area occupied by Iba-1-positive staining was elevated in KO injured animals when compared to uninjured controls (D), while stain area was not elevated in WT injured animals (E). Representative immunofluorescence is depicted for KO Sham (F top left), KO injured (F bottom left), WT Sham (F top right), and WT injured (F bottom right) in the right and left hemispheres (blue = DAPI, green = Iba-1). Scale bars = 100μm, 20x. Error bars = ±SD. * p<0.05, ** p<0.01.