Hippocampal injury.

Injured Prkdc KO (A) and WT (B) rats both exhibited reductions in right hippocampal area. Both KO (C) and WT (D) injured rats also display reductions in the number of neurons in the right CA1 pyramidal layer. Representative immunofluorescence in CA1 of KO Sham (E top left), KO injured (E bottom left), WT Sham (E top right), and WT injured (E bottom right) in the right and left hemispheres. Where neuronal loss was observed, astrogliosis was evident (green = NeuN, red = GFAP). (F) Measurement locations for CA1 neuronal counts (red boxes) are shown for the left and right hemispheres; A trend toward reduction in neurons in the right CA3 pyramidal layer was observed in KO (G), but not in WT (H) injured rats. Representative immunofluorescence in CA3 of KO Sham (I top left), KO injured (I bottom left), WT Sham (I top right), and WT injured (I bottom right) in the right and left hemispheres. Gliosis is also evident in these injured animals (green = NeuN, red = GFAP). (J) Measurement locations for CA3 neuronal counts (red boxes) are shown for the left and right hemispheres; Scale bars = 100μm, 20x. Error bars = ±SD. * p<0.05, ** p<0.01, ***p<0.001.