Additional file 1: of Salt-inducible expression of OsJAZ8 improves resilience against salt-stress

Figure S1. The phylogenetic tree of selected rice stress responsive C2H2-type zinc finger proteins and STZ/ZAT10 [63, 64]. Figure S2. Multiple sequence alignment of amino acid sequences of rice stress-responsive C2H2-type zinc finger proteins with STZ/ZAT10. Figure S3. Localization study of ZOS3–11 and ZOS3–12 in BY2 cells. Figure S4. DNA-binding capacity of ZOS3–11 and ZOS3–12. Figure S5. Confirmation of T-DNA inserts in BY-2 transgenic cell lines by PCR. Figure S6. Relative gene expression of OsJAZ8 in transgenic BY-2 lines ZOS3–11::JAZ8 and OsJAZ8ΔC in ZOS3–11::JAZ8ΔC. Figure S7. Dual luciferase assay for measuring ZOS3–11 promoter activity after salt and MeJA treatment. Figure S8. Percentage of filled grain in transgenic rice plants. Figure S9. Phenotypic observation of third leaf of 10 days old WT and transgenic rice plants subjected to 100 mM salt. Figure S10. Schematic diagram the constructs used for transformation. Table S11. List of PCR primers with used for Gateway cloning. Table S12. List of primers for checking T-DNA inserts. Table S13. List of primers used for qPCR. (PPTX 29561 kb