Additional file 7: of Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte

Figure S6. Expression analysis of ZxNAC083 and ZxNAC035. (a-b) qRT-PCR validation of RNA sequencing data in Z. xanthoxylum roots under osmotic stress or salt treatment for 6 h. (c) Semi-quantitative RT-PCR assay showing overexpression of ZxNAC083 in transgenic Arabidopsis plants compared to Col-0 wild-type. ZxNAC083 (20 cycles) and AtActin2 (19 cycles). (d) Semi-quantiative RT-PCR experiment assay showing overexpression of ZxNAC035 in transgenic Arabidopsis plants compared to Col-0 wildtype. ZxNAC035 (25 cycles) and AtActin2 (19 cycles). (JPG 429 kb