Additional file 2: Figure S5. of The sucrose non-fermenting 1-related kinase 2 gene SAPK9 improves drought tolerance and grain yield in rice by modulating cellular osmotic potential, stomatal closure and stress-responsive gene expression

Schematic representation of the genetic constructs based on pCAMBIA1301 plasmid used for Agrobacterium-mediated transformation of drought-sensitive indica rice cultivar IR20. (A) The gene overexpression (OE) construct of SAPK9 carrying 1086 bp CDS from O. rufipogon. The developed transgenic rice lines were designated as SAOE#1, 2, 3 etc. (B) The RNAi-mediated gene silencing (RNAi) construct of endogenous SAPK9 gene. The 605 bp 5′-part of SAPK9 CDS from O. rufipogon was cloned in sense and antisense orientation flanking an arbitrary 200 bp DNA linker. The developed transgenic rice lines were designated as RNAi#1, 2, 3 etc. (TIF 453 kb