The allosteric coupling response (<i>CR</i>) of the comprehensive ensemble model.

(a,b) CR as a function of Δgint,R and Δgint,T (in kcal/mol) when the other parameters are fixed (chosen to produce notable allostery) as: ΔGR1 = −1.0, ΔGR2 = 1.3, ΔGRT1 = 1.0, ΔGRT2 = 3.0 (all in units of kcal/mol). Note that for A-T binding mode there is no activated allosteric effect. (c,d) Distribution of CR when the stability free-energy parameters (ΔGR1, ΔGR2, ΔGRT1, ΔGRT2, Δgint,R, Δgint,T) vary randomly with an equal probability density between −8 and +8 kcal/mol. The A-R binding mode is adopted in (a,c) and the A-T binding mode is adopted in (b,d) with ΔgLig,A = −3 cal/mol.