Interplay between different states and subsystems/pathways in the comprehensive ensemble model.

The A-R binding mode is adopted. (a) Proportion of four categories [single subsystem with single pathways (S,S), single subsystem with mixing pathways (S,M), mixing subsystems with single pathways (M,S), and mixing subsystems with mixing pathways (M,M)] in systems with CR. (b) The proportion of systems with two-state transition. (c) Distribution of PRR and state probability of three subsystems (PRR + PTT for the MWC pathway, PRR + PIR + PRI + PII for the EAM pathway, and PRR + PTI + PIT for the Other pathway) for systems with CR ≈ 0.16. ΔGmax = 3 or 13 kcal/mol. The theoretical CR ~ PRR curve (blue line) for the two-state model is also plotted using Eq (9). The horizontal dashed line represents CR = 0.16.