Additional file 1: of Antigen-specific CD8+ memory stem T cells generated from human peripheral blood effectively eradicate allogeneic targets in mice

Figure S1. Enrichment of TSCM cells by differentiation inhibitors and the lymphocyte distribution changes in the cultural bulks over the TSCM preparation course. A, B TWS119 exhibited concentration-dependent enrichment of TSCM in the alloreactive co-culture, 5 μM of TWS119 was the best concentration. TSCM cells in frequency (A) and in absolute number (B) were shown. C Differentiation inhibition by rapamycin was more likely to enrich TCM instead of TSCM. D–F After allogeneic activation, differentiation inhibition, proliferation sorting, and cytokine expansion, lymphocyte distribution in the cultural bulks was revealed by FCM over the TSCM preparation course. CD3 + CD4+ T cells, CD3 + CD8 + T cells and CD3- cells in the cultural bulks were detected, and the proportion of CD3 + CD8+ T cells was increased over time (D). The majority of cultural bulks were CD3 + CD8+ TSCM (60.1 ± 11.2%) on day 14 (E). The proportion of CD3 + CD8+ TSCM in CD8+ T cells was increased over time, about 80% of CD8+ T cells were TSCM on day 14 (F). Data are represented as mean ± SD of four individual experiments. (JPG 170 kb