Additional file 1: Figure S1. of Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34+ cells of juvenile myelomonocytic leukemia

Effects of GMR CAR T cells on GM or erythroid colony growth of CB and BM CD34+ cells. Either GMR CAR T cells or mock T cells were incubated with normal CB CD34+ cells (n = 3) or normal BM CD34+ cells (n = 3) at E:T ratios of 1:1 and 1:4, in the presence of SCF + TPO + IL-3 for 2 days. Cells were then cultured on methylcellulose in media supplemented with GM-CSF, SCF, IL-3, and erythropoietin. After 14 days, the numbers of GM colonies and erythroid colonies were calculated for each. Values are expressed as percentages of the total colony numbers obtained by culture in the absence of T cells. The numbers of GM colonies grown by 500 CB CD34+ cells and BM CD34+ cells in the absence of T cells were 38.6 ± 4.6 and 24.5 ± 2.9, respectively. The numbers of erythroid colonies grown by 500 CB CD34+ cells and BM CD34+ cells in the absence of T cells were 42.7 ± 15.5 and 19.1 ± 2.6, respectively. (EPS 439 kb