Additional file 4: Figure S4. of Differential ability of MSCs isolated from placenta and cord as feeders for supporting ex vivo expansion of umbilical cord blood derived CD34+ cells

P-MSCs favors the expansion of CD34+ cells in a contact dependent manner. a Significant reduction in total nucleated cells (TNC) was observed in non-contact cultures of P-MSCs while no effect was seen in non-contact cultures of C-MSCs. b Non-contact cultures of P-MSCs displayed reduction in the percentage of CD34+ and CD34+CD38− cells as compared to contact cultures. c Reduction in the yield of TNC and CD34+ cells was due to an increase in the apoptosis in non-contact cultures as seen by a decrease in the annexinV−PI− cells in non-contact cultures. d No change in the proliferation of CD34+ cells either singly blocked with CD49d (VLA-4), CD49e (VLA-5), and CXCR-4 or in combination with CD49d + CD49e and CD49d + CD49e + CXCR-4 was observed after co-culturing with C-MSCs for 96 hrs as assessed by MTT assay. e Drastic reduction was seen in the proliferation of CD34+ cells upon blocking with CD49d + CD49e, CXCR-4, and CD49d + CD49e + CXCR-4 and co-culturing with P-MSCs. f Clonogenecity of the cells expanded on C-MSCs remained unaltered even after blocking with anti-bodies. g Significant reduction was observed in the progenitor content after blocking the CD34+ cells with the combination of antibodies and co-culture on P-MSCs. h Percentage of live population remains unchanged in the blocked set as opposed to the unblocked set in the co-cultures comprised of C-MSCs. i Viability of the expanded cells blocked with integrins and CXCR-4 reduced significantly in co-cultures with P-MSCs as feeders. Data are represented as mean ± standard deviation from three different independent experimental sets. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. (TIFF 4452 kb