Lower mutation frequency on silenced alleles following 96-hour exposure.

(A) Allele-specific mutation frequencies for KvDMR sgRNAs #1–3 in cells harvested 96 hours post transfection, following selection of transfectants in puromycin. Error bars represent SD of three biological replicates; p-values denote two-tailed paired t tests of difference between maternal and paternal alleles. *p p p S1 Data, and details of MiSeq libraries including SRA accessions are provided in S2 Data. Mat, maternal; ND, methylation analysis not done; Pat, paternal; sgRNA, single guide RNA; SRA, Sequence Read Archive.