(A) 10 (2)mbn cells were treated with either double-stranded RNA to control (Ndfip2) or dLKR/SDH for 48 h

Cells were then treated with ethanol (0) or ecdysone. ChIP assays were performed as outlined in . RNA was purified from the first extraction step and converted to cDNA, and the levels of dLKR/SDH transcript were detected by RT-PCR (dLKR/SDH transcript). (B) Antibody-enriched genomic DNA used in A and input genomic DNA per treatment were used in semiquantitative real-time PCR. Relative levels were determined by comparing the levels of DNA from the immunoprecipitated samples to the corresponding input genomic DNA. Error bars represent SEM.<p><b>Copyright information:</b></p><p>Taken from "dLKR/SDH regulates hormone-mediated histone arginine methylation and transcription of cell death genes"</p><p></p><p>The Journal of Cell Biology 2008;182(3):481-495.</p><p>Published online 11 Aug 2008</p><p>PMCID:PMC2500134.</p><p>