Methylation of dCas9-MC/MN and associated variants as assessed by FspI restriction protection assay.
- Tina Xiong (6095357)
- Dahlia Rohm (6095360)
- Rachael E. Workman (6095363)
- Lauren Roundtree (6095366)
- Carl D. Novina (200428)
- Winston Timp (6095369)
- Marc Ostermeier (110606)
Publication date
December 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
sgRNA1 was used to target methylation to site 1. Methylation was assayed from plasmid DNA extracted from ER2267 cells expressing the dCas9-MC/MN (WT) or the indicated dCas9-MC/MN variant. The first row indicates the position mutated, and the second row indicates the mutation made. The PFCSY set of mutations is in the final lane.</p
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