Light Sheet microscopy of Astrocytes.avi

Superresolved light sheet microscopy of mouse brain Astrocytes A 100µm thick block of cleared mouse brain embedded in a pluronic gel. The astrocytes are anti-GFAP/CY3 stained. XY Scales : 2mmX1.8mm for low resolution.450 µmX340µm for medium resolution.220µmX180µm for High resolutionSuperresolved sheet thickness around 1µm. In addition to astrocytes some amyloid plaques (better shown at low magnification) are imaged via their endofluoresecence. The animal model is a 12 months old cleared AD mouse (APP:PS1-21 lines).