EBV-miR-BART1-5P regulates the AMPK/mTOR/HIF1 signaling pathways.

(A) mTOR, p-mTOR and S6K1 were detected by western blot in CNE1 and HONE1 cells transfected with EBV-miR-BART1-5P mimic or co-transfected EBV-miR-BART1-5P mimic and PTEN plasmid. HONE-BART1-5P and CNE1-BART1-5P cells treated with anti-miR or anti-miR and si-PTEN were also performed. GAPDH was used as a loading control. (B) Western blot of endogenous AMPKα1, mTOR, p- mTOR, S6K1, VEGF, HIF-1α, GLUT1, LDHA, CCND1, P27 and p21 protein expression levels in HONE1 and CNE1 cells treated with si-AMPKα1 or si-control. β-actin was used as a loading control. (C) GLUT1, LDHA and HIF-1α expression was detected using immunohistochemistry assay in tumour tissues derived from tumorigenesis in nude mice models. Magnification, ×400.