Silencing of endogenous EBV-miR-BART1-5P attenuates the phenotypes in EBV-positive NPC cells.

(A) Lactate production and glucose consumption, (B) CAM angiogenesis, (C) colony formation detected in HONE-EBV cells after transfection anti-control, anti-miR, anti-miR+si-AMPKα1, anti-miR+si-PTEN and anti-miR+si-AMPKα1+si-PTEN, respectively. The data were shown as the mean ± s.e.m. (*PD) AMPKα1, mTOR, p- mTOR, S6K1, VEGF, HIF-1α, GLUT1, LDHA, CCND1, P27 and p21 protein expression levels in HONE-EBV cells treated with anti-control, anti-miR, anti-miR+si-AMPKα1, anti-miR+si-PTEN and anti-miR+si-AMPKα1+si-PTEN, respectively. β-actin was used as a loading control. (E) A proposed model demonstrating the role of EBV-miR-BART1-5P in the glycolysis, angiogenesis and proliferation of NPC cells.