Effect of MMP1 on <i>E</i>. <i>faecalis</i> growth.

Growth curves of E. faecalis in presence of active MMP1, BHI media, and protein buffer for (a) FA2-2 and (b) V583 strains respectively. Symbols represent data points. Solid lines are fits to the logistic equation of bacterial growth, y = a/{1+bexp(−kt)}, where k represents the growth rate. Error bars on the data points represent the standard deviations of 6 technical repeats. For FA2-2 strain, the best fit parameters are: a = 0.85±0.01, b = 24.98±4.44, k = 1.38±0.08 (BHI media); a = 0.81±0.01, b = 32.53±7.02, k = 1.49±0.09 (protein buffer); a = 0.80±0.01, b = 36.62±8.88, k = 1.53±0.10 (inactive MMP1); a = 0.71±0.02, b = 8.47±1.22, k = 0.79±0.06 (active MMP1). For V583 strain, the best fit parameters are: a = 0.96±0.01, b = 13.41±1.20, k = 1.05±0.04 (BHI media); a = 0.92±0.01, b = 7.70±0.62, k = 0.85±0.04 (protein buffer); a = 0.92±0.01, b = 7.73±0.65, k = 0.88±0.04 (inactive MMP1); a = 0.75±0.01, b = 10.12±1.21, k = 0.96±0.05 (MMP1). For fit parameters, error bars represent the standard error of the mean.