EfaCBA, MntH1 and MntH2 are the main Mn transporters of E. faecalis.

Cristina Colomer-Winter (697911)
Ana L. Flores-Mireles (5775419)
Shannon P. Baker (5775422)
Kristi L. Frank (160019)
Aaron J. L. Lynch (5775425)
Scott J. Hultgren (243893)
Todd Kitten (288404)
José A. Lemos (239435)

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Publication date

September 2018

DOI

10.1371/journal.ppat.1007102.g002

Publisher

Public Library of Science (PLoS)

Abstract

Cellular Mn and Fe quantifications of E. faecalis OG1RF with (A) single Δefa, ΔmntH1, ΔmntH2, or (B) double ΔefaΔmntH1, ΔefaΔmntH2, ΔmntH1ΔmntH2 and triple ΔefaΔmntH1ΔmntH2 mutant strains. Strains were grown to mid-log phase (OD600 ~ 0.5) in BHI prior to analysis. The bar graphs show the average and standard deviations of three independent ICP-OES analyses. BDL: below detection limit. Statistically significant differences were determined via ordinary one-way ANOVA with Dunnett’s multiple comparison post-test (*p ≤ 0.05, ** p ≤ 0.01, ***p ≤ 0.001, **** p ≤ 0.0001).</p