Histological and immunohistochemistry analyses of the prostate from <i>R26hAR</i>^<i>L/wt</i>:<i>PB-Cre4</i>, <i>p16</i>^<i>L/L</i>:<i>PB-Cre4</i> and <i>R26hAR</i>^<i>L/wt</i>:<i>p16</i>^<i>L/L</i>:<i>PB-Cre4</i>.

(A-B) Representative H&E images of the prostate tissue was shown for AP, DLP, and VP for R26hARL/wt:PB-Cre4 (A1-A3, magnification A1’-A3’) and R26hARL/wt:p16L/L:PB-Cre4 mice (B1-B3, magnification B1’-B3’). Scale bar used was 100 μm or 25 μm. (C). Representative H&E and IHC images of adjacent prostate tissue sections of AP from the p16L/L:PB-Cre4 mice were shown for staining with different antibodies as labeled in right bottom corner. (D). Representative H&E and IHC images of adjacent prostate tissue sections from the p16L/L: R26hARL/wt:PB-Cre4 mice were shown for staining with different antibodies (please see S1B and S1B’ Fig for positive staining of synaptophysin). Scale bar used was at 25 μm for all panels (C1-D9). (E) Pathological abnormalities of R26hARL/wt:PB-Cre4, p16L/L:PB-Cre4, and R26hARL/wt:p16L/L: PB-Cre4 mice reported for different time points.