CD166 expression is upregulated in castrated prostate epithelium and CD166 can be used to enrich stem/progenitor cells in WT mice prostate.

<p>(A) Top: Comparison of p63 (red) and CD166 (green) co-IF staining between prostate proximal region and distal region. Bottom: IHC for CD166 expression from intact vs. castrated mouse prostate. Scale bar: 50 µm. (B) Lin^-;CD166^hi, Lin^-;CD166^lo, LSC^hi;CD166^hi, and LSC^hi;CD166^lo cells were isolated by FACS from 8- to 12-week-old mice. Graph shows the percentage of sphere-forming cells, based on the spheres from each population per 2500 cells plated after 8 days of growth. Data shown as mean +/− STD (**, p<0.001, n = 3). (C) Fold change of LSC^hi;CD166^hi content based on intact WT from FACS analysis (*, p<0.05, n = 3).