rs6651252 demarcates a Wnt-responsive DNA enhancer element.

(A). Diagram of luciferase reporter vectors used in (B) and (C). The pGL3-promoter vector backbone served as a control (control-luc). A 555 bp DNA fragment encompassing rs6651252 was inserted upstream of the SV40 promoter in the pGL3-promoter plasmid vector. Grey vertical lines depict consensus TCF motifs with an asterisk depicting rs6651252. (B) and (C). Luciferase assays conducted in the indicated cell lines with values obtained from rs6651252-luc transfected cells normalized to values obtained from cells transfected with control-luc (D). Diagram of rs6651252 luciferase vectors used in (E) and (F). The T variant of rs6651252 was changed to the C variant using site-directed mutagenesis. (E) and (F). As in (B) and (C), except the indicated cells were transfected with rs6651252 harboring either ancestral T or disease-associated C variant. Error bars are SEM (***P <0.001).