Figure S5. Comparison of two biological replicates shows that 2C-ChIP is highly reproducible. The uninduced (0âh) and RA-induced (3d) 2C-ChIP data from set A and B NT2-D1 cells are highly correlated except for Ash2L and UTX, which display high background levels in negative control regions (Additional file 6: BED file 11, 13, 27, 29). (PDF 434 kb
ChIP enrichment analyzed by qPCR. The ChIP DNA was analyzed by qPCR in a region of constitutively ac...
The mean absolute difference between cancer and MSC expression of samples versus the technical varia...
<p>Chromatin immunoprecipitation (ChIP) was utilized to confirm MUC1.CT and ZEB1 occupancy at the mi...
Figure S6. 2C-ChIP results for Ash2L, CTCF, and UTX in set A. Data is displayed as outlined in Fig. ...
BED files. This folder contains the input-normalized 2C-ChIP data in BedGraph format of all the ChIP...
Figure S2. Defining the optimal 2C-ChIP linear detection range. a Diagram of the HOXA cluster region...
Figure S3. The basal and 3-day induced gene expression levels from two biological replicates correla...
Figure S1. SUZ12 binding analysis by 2C-ChIP and ChIP-qPCR correlate well at the HOXA cluster. a 2C-...
Table S5. List of primer sequences used to amplify 2C-ChIP libraries prior to deep sequencing on a P...
Table S6. List of primer sequences used for chromatin conformation capture carbon copy (5C). (XLSX 1...
Table S1. HOXA and lncRNA gene expression in NT2-D1 cells before and after a 3-day treatment with re...
5C datasets. This folder contains the read count-normalized 5C data in matrix format (.txt) of all t...
Figure S7. RA-induced NT2-D1 differentiation is accompanied by extensive conformational and epigenom...
Table S3. List of primer sequences used to PCR-quantify DNA regions isolated by chromatin immunoprec...
Abstract Background Understanding how transcription occurs requires the integration of genome-wide a...
ChIP enrichment analyzed by qPCR. The ChIP DNA was analyzed by qPCR in a region of constitutively ac...
The mean absolute difference between cancer and MSC expression of samples versus the technical varia...
<p>Chromatin immunoprecipitation (ChIP) was utilized to confirm MUC1.CT and ZEB1 occupancy at the mi...
Figure S6. 2C-ChIP results for Ash2L, CTCF, and UTX in set A. Data is displayed as outlined in Fig. ...
BED files. This folder contains the input-normalized 2C-ChIP data in BedGraph format of all the ChIP...
Figure S2. Defining the optimal 2C-ChIP linear detection range. a Diagram of the HOXA cluster region...
Figure S3. The basal and 3-day induced gene expression levels from two biological replicates correla...
Figure S1. SUZ12 binding analysis by 2C-ChIP and ChIP-qPCR correlate well at the HOXA cluster. a 2C-...
Table S5. List of primer sequences used to amplify 2C-ChIP libraries prior to deep sequencing on a P...
Table S6. List of primer sequences used for chromatin conformation capture carbon copy (5C). (XLSX 1...
Table S1. HOXA and lncRNA gene expression in NT2-D1 cells before and after a 3-day treatment with re...
5C datasets. This folder contains the read count-normalized 5C data in matrix format (.txt) of all t...
Figure S7. RA-induced NT2-D1 differentiation is accompanied by extensive conformational and epigenom...
Table S3. List of primer sequences used to PCR-quantify DNA regions isolated by chromatin immunoprec...
Abstract Background Understanding how transcription occurs requires the integration of genome-wide a...
ChIP enrichment analyzed by qPCR. The ChIP DNA was analyzed by qPCR in a region of constitutively ac...
The mean absolute difference between cancer and MSC expression of samples versus the technical varia...
<p>Chromatin immunoprecipitation (ChIP) was utilized to confirm MUC1.CT and ZEB1 occupancy at the mi...