Model fit and predictions for intermediate media exchange frequencies.

(A—C) Undisturbed culture conditions over the 9 day period (denoted as 10 day), led to a drop in all cell populations as compared to the 2-day media exchange. Model parameters were optimized to obtain a high degree of correlation with experimental data simply by changing media frequency change in the model to 10 days. The model was able to predict the system response to a 5-day media exchange for all the 3 cell populations, without any parameter optimization. Subtle differences in cell numbers were captured over long term culture. Inset: a close up of the model and data fit between days 7–9. Error bars represent standard deviation.