Mathematical model accurately predicts dose-dependent changes in damping rate.

<p>(<b>A</b>) Example single-cell trajectories (top) and population-averaged trajectories (bottom, mean of 1,000 cells) of cells under various treatments. Cells with the nominal parameter set (left, black) closely match the experimental damping rate for unperturbed cells. Cells with simulated KL001 action (red, center) are noisier at the single-cell level, and show faster damping at the population-level. Cells with simulated longdaysin action show slightly more accurate single-cell rhythms, with a corresponding reduction in the population-level damping rate. (<b>B</b>) The model accurately predicts the general trend of period vs. damping rate for both KL001 and longdaysin perturbations. Experimental data points represent the mean of two replications at each concentration. Computational data points represent the mean of ten independent population simulations.