Saa regulates neutrophil abundance and maturation.

(A) Flow cytometry analysis of lyz:EGFP+ neutrophil abundance from whole 6 dpf WT and saa mutant zebrafish larvae (results are combined from 3 independent experiments, ≥ 4 replicates / genotype / experiment, 60–90 larvae / replicate). (B-C) Morphological analysis of lyz:DsRed+ neutrophil cytospins stained with Wright-Giemsa from adult WT and saa mutant zebrafish kidneys (5–6 adult zebrafish kidneys / genotype / experiment, 2 independent experiments, n ≥ 199 cells analyzed / genotype / experiment) (scale bar = 10 μm). (D-F) qRT-PCR of leukocyte-associated transcripts lysozyme C (lyz), l-plastin (lcp), and colony-stimulating factor 3a (csf3a) from 6 dpf whole zebrafish larvae (n = 4 replicates / genotype / timepoint, 25–30 larvae / replicate). Data in panel A analyzed by t-test. Data in panel C analyzed by chi-squared test. Data in panels D-F analyzed by one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as mean ± SEM. * p p p 0.001, **** p 0.0001.