Transient expression of the <i>lyz</i>:MPO-mEmerald transgene labels zebrafish neutrophils.
- Kyle D. Buchan (6616364)
- Tomasz K. Prajsnar (375674)
- Nikolay V. Ogryzko (6616367)
- Nienke W. M. de Jong (696604)
- Michiel van Gent (525399)
- Julia Kolata (6616370)
- Simon J. Foster (332978)
- Jos A. G. van Strijp (176548)
- Stephen A. Renshaw (232640)
Publication date
April 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
A) Schematic of the lyz:MPO-mEmerald cmlc2:EGFP construct, which includes the neutrophil-specific promoter (lyz), the MPO gene with a C-terminal fluorescent tag (MPO-mEmerald) and a green heart marker to aid optimisation of transgenesis (cmlc2:EGFP). B) A zebrafish larva at 3 days post fertilisation (dpf), with the caudal haematopoietic tissue (CHT) indicated by the red box. C) The CHT of a double-transgenic Transient lyz:MPO-mEmerald,cmlc2:EGFP; Tg(lyz:nfsB-mCherry)sh260 larva with a population of neutrophils expressing both mEmerald and mCherry. The white arrowhead indicates the neutrophil enlarged below. D) Enlarged view of a neutrophil expressing mEmerald and mCherry.</p
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