<p><b>A</b>) T-MPO target sequence and corresponding mutated sequence of the <i>in vitro</i> synthesized CD mRNA. <b>B</b>) Western blotting of CD in homogenates of 2 dpf embryos and 4 dpf larvae deriving from control injection (CTRL), injection with T-MPO alone (T-MPO) and injection with T-MPO plus 200 pg/egg of mutant CD mRNA (RESCUED). The filter was incubated with polyclonal antibody against rat CD, stripped and re-probed with monoclonal antibody against β-actin. The densitometry ratio of CD <i>vs</i> actin expression is shown. (a.u. = arbitrary units). Data reproduced in two to three independent experiments. The empty arrow points to CD expressed by the exogenous mRNA. <b>C</b>) Representative image of 4 dpf larvae obtained from contr...
<p>(A) The morphologies of zebrafish morphants at 72 hpf. Note that the morphants of <i>ttc4</i>, <i...
<p>Morpholinos against exon 6 and exon 8 of <i>zABCB6</i> and wildtype <i>zABCB6</i> mRNA transcript...
<p>A. Diagrams of plasmid constructs. In pFL-GIC the core cds and GFP cds are driven by L-FABP enhan...
<p><b>A</b>) Schematic representation of S-MPO and T-MPO target regions in the CD RNA. The sequences...
<p>Immunofluorescence staining of CD and α-actin in eye sections derived from 4 dpf micro-injected l...
<p>Representative images of 4 dpf larvae resulting from control injection (CTRL), injection with S-M...
<p><b>A</b>) Western blotting of CD in homogenates of un-fertilized eggs (UFE), embryos (at 30% epib...
A) Schematic of the lyz:MPO-mEmerald cmlc2:EGFP construct, which includes the neutrophil-specific pr...
<p>(A) Brightfield photographs of 26 hours old <i>Tg(BRE:mRFP)</i> transgenic embryos treated with D...
CD (cathepsin D) is a ubiquitous lysosomal hydrolase involved in a variety of pathophysiological fun...
<p>(A) Western blots for MPO, TNF-α, iNOS, and β-actin proteins from adult zebrafish; (B) relative d...
The rapid embryonic development and high fecundity of zebrafish contribute to the great advantages o...
<p>(A) A pool of 28 h dpf embryos from the stable transgenic founder fish showing the EGFP expressio...
<p>(A) Confirmation of the presence of <i>col6a1</i> mRNA in different organs of adult WT zebrafish:...
(a-b) The RT-PCR (reverse transcription-PCR) results were conducted to analyze the efficiency of sb-...
<p>(A) The morphologies of zebrafish morphants at 72 hpf. Note that the morphants of <i>ttc4</i>, <i...
<p>Morpholinos against exon 6 and exon 8 of <i>zABCB6</i> and wildtype <i>zABCB6</i> mRNA transcript...
<p>A. Diagrams of plasmid constructs. In pFL-GIC the core cds and GFP cds are driven by L-FABP enhan...
<p><b>A</b>) Schematic representation of S-MPO and T-MPO target regions in the CD RNA. The sequences...
<p>Immunofluorescence staining of CD and α-actin in eye sections derived from 4 dpf micro-injected l...
<p>Representative images of 4 dpf larvae resulting from control injection (CTRL), injection with S-M...
<p><b>A</b>) Western blotting of CD in homogenates of un-fertilized eggs (UFE), embryos (at 30% epib...
A) Schematic of the lyz:MPO-mEmerald cmlc2:EGFP construct, which includes the neutrophil-specific pr...
<p>(A) Brightfield photographs of 26 hours old <i>Tg(BRE:mRFP)</i> transgenic embryos treated with D...
CD (cathepsin D) is a ubiquitous lysosomal hydrolase involved in a variety of pathophysiological fun...
<p>(A) Western blots for MPO, TNF-α, iNOS, and β-actin proteins from adult zebrafish; (B) relative d...
The rapid embryonic development and high fecundity of zebrafish contribute to the great advantages o...
<p>(A) A pool of 28 h dpf embryos from the stable transgenic founder fish showing the EGFP expressio...
<p>(A) Confirmation of the presence of <i>col6a1</i> mRNA in different organs of adult WT zebrafish:...
(a-b) The RT-PCR (reverse transcription-PCR) results were conducted to analyze the efficiency of sb-...
<p>(A) The morphologies of zebrafish morphants at 72 hpf. Note that the morphants of <i>ttc4</i>, <i...
<p>Morpholinos against exon 6 and exon 8 of <i>zABCB6</i> and wildtype <i>zABCB6</i> mRNA transcript...
<p>A. Diagrams of plasmid constructs. In pFL-GIC the core cds and GFP cds are driven by L-FABP enhan...