Saa mediates neutrophil behavior <i>in vivo</i>.

(A-B) Imaging and quantification of lyz:EGFP+ neutrophils recruited to tail wound margin over 6 hours following caudal fin amputation (dashed red line indicates wound margin; scale bar = 250 μm) (n ≥ 24 larvae / genotype at 6 hour time point). (C) Measurement of lyz:DsRed+ neutrophil speed from time-lapse imaging in caudal fin tissue over 6 hour period following amputation (n = 4 larvae / genotype, 87–112 cells tracked / genotype). (D-E) Representative images of 6 dpf Tg(lyz:DsRed) WT and saa-/- larvae (scale bar = 500 μm). (F) Enumeration of intestine-associated lyz:EGFP+ cells in 6 dpf larvae (n = 32–40 larvae / genotype). (G-J) Quantitative analysis of lyz:EGFP+ neutrophil behavior from time-lapse imaging of distinct anatomical compartments (intestine and trunk, ROIs in panel D) in 6 dpf larval zebrafish (6 larvae / genotype, ≥ 23 cells analyzed / genotype / tissue). Data analyzed by t-test. For panel B, statistical comparisons were performed within each time point. Data are presented as mean ± SEM. * p p p p 0.0001.