Immunohistochemistry.

Image (20x) of the caudate putamen of an animal that was exposed to either chronic quinpirole (QP) or functioned as a control (SAL) after staining with either the D2R antibody (top), the mGluR5 antibody (middle) or the GLT-1 antibody (bottom). As GLT-1 is present on the astrocyte, the staining will occupy the space between neuronal cells. These neuronal cells will therefore remain uncoloured. For visual representation, one exemplary neuron is marked in each staining with an arrow. In the right utmost column, the area percentage expressed as percentage of pixels in the image that have been highlighted by the staining after either QP (n = 7) or SAL (n = 7). Individual points, median and standard deviation of each group are displayed. * p < 0.05.