Characterization of 2D neuronal culture differentiated from neural stem cells.

<p>2D neurons were immunostained with antibodies against the markers NeuN, GFAP, BT3 and MAP2. Differentiated neurons from all five subjects showed NeuN-, BT3- and MAP2-positive cells. Cells positive for the astrocyte marker GFAP were also presented. The left column of images is an illustration of merged NeuN (red) and GFAP (green) staining. NeuN (red) is highly expressed in the nucleus of differentiated neurons whereas GFAP (green) is expressed in the filament of the astrocytes. Nuclei staining was illustrated in blue across all images. Scale bar: 100 μm.