Comparison of staining efficacy of samples acquired using BD LSRFortessa flow cytometer.

(A) PerCP fluorescence of unstained oocysts from an infected mouse. Alexa 488 fluorescence of stained sample from uninfected mouse (B) or stained samples from infected mice (C to F). Increasing levels of parasite burdens (counts in Y axis) show decreasing antibody staining efficacy: > 90% (C); 50% (D); 25% (E); < 25% (F).