Flow cytometric analysis of BM samples.

<p>A representative cytofluorometric analysis of a freshly isolated GD2 positive cell preparation, tested with anti-B7H3 (5B14) and anti-GD2 mAb in combination with: anti-CD45, anti-HLA-G, anti-calprotectin, anti-CD56, anti-CD117 (c-kit), anti-CD37 and anti-CD177. The first plot shows anti-B7H3 specific mAb fluorescence intensity (Y axis) <i>versus</i> side-scatter (X axis), whereas all the other plots show specific mAb fluorescence intensity (Y axes) <i>versus</i> anti-GD2 specific mAb fluorescence intensity (X axes). Letters indicated in each panel are assigned from Kaluza software to each different single sample analysis. ⁺⁺ indicates double positive cells (upper right panel), ⁺⁻ and ⁻⁺ indicate single positive cells (upper left and lower right panels), ⁻⁻ indicates double negative cells (lower left panel). Percentages of single positive (first plot) or double positive (all the other plots) cells are indicated.