Comparison of unstained and stained samples acquired using BD LSRFortessa, BD LSRFortessa X-20 and BD FACSCanto II flow cytometers.

Unstained samples from the same source acquired using BD LSRFortessa (A), BD LSRFortessa X-20 (B) and BD FACSCanto II (C) flow cytometers. Stained samples from the same source acquired using BD LSRFortessa (D), BD LSRFortessa X-20 (E) and BD FACSCanto II (F) flow cytometers. Regardless of the flow cytometer used for acquisition, approximately half of the number of events in the Oocyst gate of unstained oocysts are present in the Oocyst gate of stained oocysts due to loss during the antibody staining procedure.