<i>Hdh</i>^neoQ20/<i>Hdh</i>^ex4/5, <i>Hdh</i>^neoQ50/<i>Hdh</i>^ex4/5 and <i>Hdh</i>^neoQ111/<i>Hdh</i>^ex4/5 hypomorphic embryos.

A) Representative pictures of wild-type (WT), Hdhex4/5/+, HdhneoQ20/+, HdhneoQ50/+, HdhneoQ111/+, HdhneoQ20/Hdhex4/5, HdhneoQ50/Hdhex4/5 and HdhneoQ111/Hdhex4/5 embryos. The size and the shape of embryos with different genotypes is shown at E18.5 for animals generated by crossing HdhneoQ20/+ with Hdhex4/5/+ mice and HdhneoQ50/+ with Hdhex4/5/+ mice. For embryos generated by crossing HdhneoQ111/+ with Hdhex4/5/+ mice, representative pictures were taken at E 8.5. Dashed white line in the WT image outlines the rostral part of the forebrain headfolds and the white arrow points to the heart. The yellow dashed lines in the HdhneoQ111/Hdhex4/5 images depict anterior endoderm in the late stage streak embryo, typical of a mouse developmental stage of E7.5. Scale bar = 300μm. Tables 1–3 summarizes the appearances of all embryos analyzed and the penetrance of the phenotypes. B-C) Box-and-whisker graphs show quartiles of height in cm (cm) and weight in grams (g) of different genotypic groups of animals generated by crossing HdhneoQ20/+ with Hdhex4/5/+ mice. The median is indicated and whiskers indicate 1.5XIQR. The number (N) of animals for each group is indicated above. Error bars represent standard deviations from the mean. D-E) Box-and-whisker graphs show quartiles of height in cm (cm) and weight in grams (g) of different genotypic groups of animals generated by crossing HdhneoQ50/+ with Hdhex4/5/+ mice. The median is indicated and whiskers indicate 1.5XIQR. The number (N) of animals for each group is indicated above. A significant reduction in size of HdhneoQ50/Hdhex4/5 embryos is noted. P value < 0.05 (*). Error bars represent standard deviations from the mean.