Embryos from HFD mice experience a higher frequency of degradation and delayed developmental progression.

<p>[A] Representative image of embryos collected from control and HFD mice at 46 h post-hCG. Red and black arrowheads indicate degraded and 1-cell embryos, respectively. Scale bar, 100 µm. [B] Quantification of 1-cell, 2-cell and degraded embryos from control and HFD mice [n = 110 control embryos from 6 mice and n = 184 HFD embryos from 9 mice, pooled from three replicates]. [C] Histogram showing the blastocyst formation rate of 2-cell embryos from control and HFD mice. [n = 90 for control and n = 65 for HFD pooled from three replicates]. Error bars indicate ± sd. *, P<0.05 vs. control.