Different contribution of ERMES components to peroxisome population.

(A) Percentage of cells for a given number of peroxisomes per cell is shown for the wild type and the four ERMES mutant strains grown on glucose. For each strain, the number of peroxisomes per cell was counted from images of two counts of at least 100 non-budding cells from three independent experiments. Bars represent the standard deviation (SD). On each graph, the average number (± SD) of peroxisomes is indicated. (*) indicates that the difference between a mutant and the reference strain (BY4741) is significant after statistical analyses (Student’s t test, p-values < 0.005). (B) Wild-type and ERMES mutant cells expressing a Ds.Red protein targeted to the peroxisomal matrix (Ds.Red-SKL) were analyzed by fluorescence microscopy. Typical views of the five different strains are shown (scale bar 5 μm).