The effect of overproduction of Pex11 family members on peroxisome proliferation.

<p>Representative CLSM images of <i>P. chrysogenum</i> GFP.SKL cells: WT (<b>A</b>); overproducing Pex11 (<b>B</b>), Pex11B (<b>C</b>) or Pex11C (<b>D</b>). Cells were grown for 40 h in PPM. Scale bars represent 5 µm. <b>E</b>. Western blot showing overproduction of Pex11 (top), Pex11B, (center) or Pex11C (bottom). Crude extracts of DS17690 and strains overproducing Pex11 proteins were used for SDS-PAGE and western blotting and probed with specific antisera. Equal amounts of protein were loaded per lane. <b>F</b>. Electron micrograph of <i>P. chrysogenum</i> cells overproducing Pex11B. P-peroxisome; M-mitochondrion. Scale bar represents 1 µm.