Figure S14. Multidimensional scaling (MDS) plot depicting Euclidean distances (similarities) between RNA-sequencing samples. MDS plot shows relative similarities between RWPE-1, TET2-KO cells, and the prostate adenocarcinoma 22Rv1 cell line based on RNA sequencing results. (TIF 13562 kb
Figure S7. Pathway enrichment analysis of genes losing expression in CRISPR-TET2 knockout cells. Vis...
Figure S2. Off-target analysis of CRISPR-TET2 clones (CR1 and CR2). Sanger sequencing chromatograms ...
Figure S6. Heatmap showing expression of 780 genes which exhibited significant expression alteration...
Figure S13. Diagnostic enrichment plot of MBD-Seq samples from TET2-KO cells as compared to input co...
Table S6. Analysis of candidate gene probes in an independent methylation array dataset (GSE73549). ...
Figure S10. Heatmap showing methylation of seven candidate genes for all tumor (n = 428) and normal ...
Table S2. Genes exhibiting both significantly increased promoter methylation and significantly lower...
Table S1. Primer and probe sequences used for CRISPR verification and methylation-specific qPCR. (XL...
Table S7. Clinicopathological characteristics of prostate tumor tissues used for methylation analysi...
Figure S1. CRISPR-TET2 guide RNA targets the first coding exon of TET2. Arrows indicate the CRISPR t...
Figure S9. Methylation array probe locations on candidate genes with respect to the TET2-knockout me...
Figure S3. Overexposed Western blot for TET2 protein in parental RWPE-1 and TET2-KO cells. Overexpos...
Table S3. Characteristics of the 61 genes exhibiting significantly increased promoter methylation an...
Table S5. Association of candidate gene probe methylation with ERG fusion status. (XLSX 19 kb
Figure S5. Heatmap showing expression of all differentially expressed and downregulated genes in bot...
Figure S7. Pathway enrichment analysis of genes losing expression in CRISPR-TET2 knockout cells. Vis...
Figure S2. Off-target analysis of CRISPR-TET2 clones (CR1 and CR2). Sanger sequencing chromatograms ...
Figure S6. Heatmap showing expression of 780 genes which exhibited significant expression alteration...
Figure S13. Diagnostic enrichment plot of MBD-Seq samples from TET2-KO cells as compared to input co...
Table S6. Analysis of candidate gene probes in an independent methylation array dataset (GSE73549). ...
Figure S10. Heatmap showing methylation of seven candidate genes for all tumor (n = 428) and normal ...
Table S2. Genes exhibiting both significantly increased promoter methylation and significantly lower...
Table S1. Primer and probe sequences used for CRISPR verification and methylation-specific qPCR. (XL...
Table S7. Clinicopathological characteristics of prostate tumor tissues used for methylation analysi...
Figure S1. CRISPR-TET2 guide RNA targets the first coding exon of TET2. Arrows indicate the CRISPR t...
Figure S9. Methylation array probe locations on candidate genes with respect to the TET2-knockout me...
Figure S3. Overexposed Western blot for TET2 protein in parental RWPE-1 and TET2-KO cells. Overexpos...
Table S3. Characteristics of the 61 genes exhibiting significantly increased promoter methylation an...
Table S5. Association of candidate gene probe methylation with ERG fusion status. (XLSX 19 kb
Figure S5. Heatmap showing expression of all differentially expressed and downregulated genes in bot...
Figure S7. Pathway enrichment analysis of genes losing expression in CRISPR-TET2 knockout cells. Vis...
Figure S2. Off-target analysis of CRISPR-TET2 clones (CR1 and CR2). Sanger sequencing chromatograms ...
Figure S6. Heatmap showing expression of 780 genes which exhibited significant expression alteration...